Hopefully you never have to, but just in case you have to run run 16-bit applications on Windows 10 64-bit in #WindowsVirtualDesktop (#WVD) then this article provides a way to do it.

Read more here: https://techcommunity.microsoft.com/t5/windows-dev-appconsult/running-16-bit-applications-on-windows-10-64-bit/ba-p/1671418